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Abstracts/Presentation Description
Wing Kit Lam1, Christina Pui Ying Fan1, Winnie Yim Fong Law1, Tsz Fung Wong1, Sze Fai Yip1
1Department of Clinical Pathology, Tuen Mun Hospital
1Department of Clinical Pathology, Tuen Mun Hospital
Background: The immunochromatographic strip test (ICT) based on detection of Hb Bart’s and the recently described α-thalassaemia early eluting peak (αEEP) in Variant II high-performance liquid chromatography are potential methods for α-thalassaemia screening1,2. This study evaluated the stability and potential interferences of αEEP and ICT to assess the potential pitfalls and suitability of routine laboratory use.
Methods: The αEEP and ICT were assessed for stability after 14 days of storage. Interference assessment of αEEP and ICT by icterus, lipaemia, and saline washing were performed by spiking experiments followed by saline washing. The performance of αEEP and ICT were assessed by comparing with genotyping results in 21 samples with glycated Hb peak (P2) ≥ 6.0%.
Results: Both αEEP and ICT were stable up to 14 days, and showed no interference by bilirubin up to 1086 µmol/L and by triglyceride up to 61.32 mmol/L. In the 21 cases with P2 ≥ 6.0%, the sensitivity of αEEP and ICT for detecting --SEA were 100% and 90.9%, respectively.
Conclusion: Both αEEP and ICT results are stable and robust to interferents including icterus, lipaemia and high glycated haemoglobin level. Both tests are suitable for application in routine diagnostic laboratories for α-thalassaemia detection.
Methods: The αEEP and ICT were assessed for stability after 14 days of storage. Interference assessment of αEEP and ICT by icterus, lipaemia, and saline washing were performed by spiking experiments followed by saline washing. The performance of αEEP and ICT were assessed by comparing with genotyping results in 21 samples with glycated Hb peak (P2) ≥ 6.0%.
Results: Both αEEP and ICT were stable up to 14 days, and showed no interference by bilirubin up to 1086 µmol/L and by triglyceride up to 61.32 mmol/L. In the 21 cases with P2 ≥ 6.0%, the sensitivity of αEEP and ICT for detecting --SEA were 100% and 90.9%, respectively.
Conclusion: Both αEEP and ICT results are stable and robust to interferents including icterus, lipaemia and high glycated haemoglobin level. Both tests are suitable for application in routine diagnostic laboratories for α-thalassaemia detection.
References:
1. Winichagoon P, Kumpan P, Holmes P, et al. Validation of the immunochromatographic strip for α-thalassemia screening: a multicenter study. Transl Res. 2015 Jun;165(6):689-95.
2. Lam WK, Law WYF, Wong TF, et al. A specific early eluting peak in Bio-Rad Variant II cation-exchange high-performance liquid chromatography (CE-HPLC) for the screening of alpha-thalassaemia in a Chinese population. Pathology. 2023 Oct;55(6):835-842.
1. Winichagoon P, Kumpan P, Holmes P, et al. Validation of the immunochromatographic strip for α-thalassemia screening: a multicenter study. Transl Res. 2015 Jun;165(6):689-95.
2. Lam WK, Law WYF, Wong TF, et al. A specific early eluting peak in Bio-Rad Variant II cation-exchange high-performance liquid chromatography (CE-HPLC) for the screening of alpha-thalassaemia in a Chinese population. Pathology. 2023 Oct;55(6):835-842.
Author Contribution Statement: Lam WK conceptualised the study, designed the study and drafted the abstract; Lam WK, Fan CPY, Law WYF, Wong TF collected and analysed the data; Yip SF analysed the data. All authors reviewed and approved the abstract.
Speaker/Presenting Authors
Authors
Submitting/Presenting Authors
Dr. Wing Kit Lam - Tuen Mun Hospital (Hong Kong SAR, China, Hong Kong SAR, China)