ePoster
100% Page: /
Abstracts/Presentation Description
Shiye Chen1, Corinna van den Heuvel1, Roger Byard11,2, Peter Blumbergs1, Jim Manavis1, Neil Langlois1,2
1University of Adelaide, South Australia, Australia
2Forensic Science SA, South Australia, Australia
Glycophorin A is present on the surface of erythrocytes; its presence can be shown using immunohistochemistry. Erythrocytes are released into the tissue following injury – such as in a bruise of the skin or contusion of the brain. It has been observed that the morphological appearance of Glycophorin A stained erythrocytes is different in bruises of different ages, but there are no published studies investigating if this can be used to assist with determining the age of an intracranial haemorrhage for forensic purposes. A pilot study was performed to address this gap in knowledge. Samples with intracranial haemorrhages of known age were used (15 intracerebral and 5 subdual cases). Glycophorin A, CD68, Heme-oxygenase-1 and Perls staining was performed. The expected appearance of granular staining of Glycophorin A did not occur in intracerebral haemorrhages but did in subdural haemorrhages. Also, less Perls staining than expected occurred in intracerebral haemorrhages. The findings of this limited, pilot study suggest Glycophorin A staining may not be useful for determine the age of intracerebral haemorrhages (due to contusions) but may be useful for subdural haemorrhages. The findings also suggest erythrocyte breakdown is different in the brain to subdural hematomas, which warrants further investigations.
Statement of Originality: Shiye Chen performed the analysis, wrote the abstract & contributed to project development.
1University of Adelaide, South Australia, Australia
2Forensic Science SA, South Australia, Australia
Glycophorin A is present on the surface of erythrocytes; its presence can be shown using immunohistochemistry. Erythrocytes are released into the tissue following injury – such as in a bruise of the skin or contusion of the brain. It has been observed that the morphological appearance of Glycophorin A stained erythrocytes is different in bruises of different ages, but there are no published studies investigating if this can be used to assist with determining the age of an intracranial haemorrhage for forensic purposes. A pilot study was performed to address this gap in knowledge. Samples with intracranial haemorrhages of known age were used (15 intracerebral and 5 subdual cases). Glycophorin A, CD68, Heme-oxygenase-1 and Perls staining was performed. The expected appearance of granular staining of Glycophorin A did not occur in intracerebral haemorrhages but did in subdural haemorrhages. Also, less Perls staining than expected occurred in intracerebral haemorrhages. The findings of this limited, pilot study suggest Glycophorin A staining may not be useful for determine the age of intracerebral haemorrhages (due to contusions) but may be useful for subdural haemorrhages. The findings also suggest erythrocyte breakdown is different in the brain to subdural hematomas, which warrants further investigations.
Statement of Originality: Shiye Chen performed the analysis, wrote the abstract & contributed to project development.
Speaker/Presenting Authors
Authors
Submitting/Presenting Authors
A/Prof Neil Langlois - University of Adelaide and Forensic Science SA (SA, Australia)